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MIDORIGreen Advance TBE Agarose Tablets – Agarose tablets with integrated DNA stain (MIDORIGreen Advance) and TBE powder; 75 pieces per box
MIDORIGreen Advance TBE Agarose Tablets – safe, clean and fast
The MIDORIGreen Agarose Tablets are a clean and fast solution for preparing agarose gels without time-consuming weighing. The agarose tablet only has to be solved in pure water. After heating and pouring of the solution the gel is ready for loading. As MIDORIGreen DNA Stain, TBE powder and agarose are integrated components, there is no addition of other ingredients necessary. In particular for researchers, who are preparing many agarose gels, this is a very comfortable and easy solution to save time. Another big advantage is the safety for the user. As a safe alternative to ethidium bromide MIDORIGreen Advance is used as DNA stain. MIDORIGreen Advance DNA Stain is non-carcinogenic – hence it is optimal for a safe work with agarose gels and no special disposing of waste is necessary.
|Gel (%)||Agarose (g)||Water (mL)||TBE (g)||TBE concentration*||MIDORI Green amount (µL)||MIDORI Green calculated amount in 100 mL gel*|
|1.0 % gel||0.5 g||50 ml||0.5 g||0.59 x||2 µl||4.00 µl|
|1.2 % gel||0.5 g||42 ml||0.5 g||0.71 x||2 µl||4.70 µl|
|1.5 % gel||0.5 g||33 ml||0.5 g||0.88 x||2 µl||6.06 µl|
|2.0 % gel||0.5 g||25 ml||0.5 g||1.18x||2 µl||8.00 µl|
*TBE is suitable to use in rage 0.5 – 1 x concentration.
**MIDORIGreen DNA stain optimum is 4-6 µl per 100 ml agarose gel.
Yes, it is exactly as you presumed. You can reduce the amount of water to the half to get a 2% agarose solution. With 2% agarose we get satisfying results. I wouldn’t recommend to increase the percentage much more than 2%, because otherwise the background could be to strong (theoretically).
In the beginning you solve the tablet in cold distilled water (water with room temperature is fine) till it is completely solved. From experience it takes around one minute. It is easy to observe the solving. After the disintegration of the tablet you heat the mixture till you can’t see any streaks. For the heating you have different options. The easiest it to choose the microwave. You will heat the mixture as long as it is boiling (around 30 sec in the microwave) to be sure to solve the agarose completely. I suppose you mean if the casted gel could be stored? Yes, that is possible but you have to ensure that the gel is wrapped in a wet tissue (and additionally with a foil so that it can’t dry out) and isn’t affected by light. You can store the gels for one week like that.
We wouldn’t recommend to use TAE buffer. The gel is a TBE gel and using TAE running buffer wouldn’t result in an optimal running behavior because of different conductivity.